ssuA

168

aliphatic sulfonate ABC transporter (binding lipoprotein)

Locus: BSU_08840

Molecular weight: 36.18 kDa

Isoelectric point: 4.85

Protein length: 332 aa

Gene length: 999 bp

Function: sulfonate uptake

Product: aliphatic sulfonate ABC transporter (binding lipoprotein)

Essential: no

Synonyms: ssuA, ygbA, yzeA

Genomic Context

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List of homologs in different organisms, belongs to COG0715 (Galperin et al., 2021)

Gene

Coordinates: 962,179 → 963,177

The protein

Protein family

• bacterial solute-binding protein ssuA/tauA family (with YtlA, according to UniProt)

Structure

• 2X26 (PDB) (from E. coli,32% identity ) PubMed
• P40400 (AlphaFold)

Localization

• associated to the membrane (via SsuC) PubMed

Expression and Regulation

Operons

• Genes: ssuB-ssuA-ssuC-ssuD-ygaN

  Description: PubMed

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A252 (ygbA::erm), available at the NBRP B. subtilis, Japan
• 1A800 ( ssuA::kan), PubMed, available at BGSC
• BKE08840 (ΔssuA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCGCTTCCGCCCTTT,  downstream forward: _UP4_CAATGATGAAAGCAGAGGCT
• BKK08840 (ΔssuA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCGCTTCCGCCCTTT,  downstream forward: _UP4_CAATGATGAAAGCAGAGGCT

References

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