sspI

168

small acid-soluble spore protein (minor), SASP

Locus: BSU_28660

Molecular weight: 7.72 kDa

Isoelectric point: 4.49

Protein length: 71 aa

Gene length: 216 bp

Function: protection of spore DNA

Product: small acid-soluble spore protein (minor), SASP

Essential: no

Synonyms: sspI, ysfA

Genomic Context

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List of homologs in different organisms, belongs to COG5856 (Galperin et al., 2021)

Gene

Coordinates: 2,931,692  2,931,907

The protein

Protein family

• sspI family (single member, according to UniProt)

Structure

• P94537 (AlphaFold)

Expression and Regulation

Operons

• Genes: sspI

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores PubMed

Biological materials

Mutant

• MGNA-B025 (ysfA::erm), available at the NBRP B. subtilis, Japan
• BKE28660 (sspI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAACGCTCCTTTTT,  downstream forward: _UP4_TAAGACATGAAACCGGGTGA
• BKK28660 (sspI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAACGCTCCTTTTT,  downstream forward: _UP4_TAAGACATGAAACCGGGTGA

References

Reviews

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Original Publications

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