sspH

168

small acid-soluble spore protein (minor)

Locus: BSU_08110

Molecular weight: 6.73 kDa

Isoelectric point: 4.46

Protein length: 59 aa

Gene length: 180 bp

Function: protection of spore DNA

Product: small acid-soluble spore protein (minor)

Essential: no

Synonyms: sspH, yfjU

Genomic Context

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List of homologs in different organisms, belongs to COG5855 (Galperin et al., 2021)

Gene

Coordinates: 885,629  885,808

The protein

Protein family

• sspH family (single member, according to UniProt)

Structure

• O31552 (AlphaFold)

Localization

• spore core (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: sspH

  Description: PubMed

  Regulation

  • repressed by glucose (3.7-fold) (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

• Genes: acoR-sspH

  Description: PubMed

  Regulation

  • repressed by glucose (3.7-fold) (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores PubMed

Biological materials

Mutant

• MGNA-C353 (yfjU::erm), available at the NBRP B. subtilis, Japan
• BKE08110 (sspH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTAACACCCTCCTTCA,  downstream forward: _UP4_TAAACAAAAATGGCCCGCTT
• BKK08110 (sspH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTAACACCCTCCTTCA,  downstream forward: _UP4_TAAACAAAAATGGCCCGCTT

References

Reviews

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Original Publications

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