sigO

168

RNA polymerase sigma factor SigO (with RsoA)

Locus: BSU_33230

Molecular weight: 22.54 kDa

Isoelectric point: 9.78

Protein length: 177 aa

Gene length: 531 bp

Function: RNA polymerase sigma factor

Product: co-sigma factor with RsoA

Essential: no

Synonyms: sigO, yvrI

Genomic Context

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List of homologs in different organisms, belongs to COG1191 (Galperin et al., 2021)

Gene

Coordinates: 3,409,462 → 3,409,992

The protein

Protein family

• Sigma-70 factor family (according to UniProt)

Domains

• contains a DNA-binding helix-turn-helix motif (HTH) and the region 4.2 of Sigma factors PubMed

Structure

• O34843 (AlphaFold)

Expression and Regulation

Operons

• Genes: sigO-rsoA

  Description: PubMed

  Regulation

  • induced by acidic growth conditions PubMed

  Regulatory mechanism

  • YvrHb: activation, PubMed, in yvrHb regulon

  Sigma factors

  • SigO: sigma factor, PubMed, in sigO regulon
  • RsoA: sigma factor, in rsoA regulon

Biological materials

Mutant

• MGNA-B052 (yvrI::erm), available at the NBRP B. subtilis, Japan
• BKE33230 (ΔsigO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCTTCATTATCTCCTTAC,  downstream forward: _UP4_CAAAAAAAGGGGGAAAAGTA
• BKK33230 (ΔsigO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCTTCATTATCTCCTTAC,  downstream forward: _UP4_CAAAAAAAGGGGGAAAAGTA

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

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