ScoA

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  • Description: probable succinyl CoA:3-oxoacid CoA-transferase (subunit A)

Gene name scoA
Synonyms yxjD
Essential no
Product probable succinyl CoA:3-oxoacid CoA-transferase (subunit A)
Function lipid metabolism
Gene expression levels in SubtiExpress: scoA
MW, pI 25 kDa, 6.613
Gene length, protein length 714 bp, 238 aa
Immediate neighbours scoB, yxjC
Sequences Protein DNA Advanced_DNA
Genetic context
ScoA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ScoA expression.png
























Categories containing this gene/protein

utilization of lipids, sporulation proteins

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

  • Locus tag: BSU38990

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA (according to Swiss-Prot)
  • Protein family: 3-oxoacid CoA-transferase subunit A family (according to Swiss-Prot)
  • Paralogous protein(s): YodS

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (CcpA) PubMed
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lucy Stols, Min Zhou, William H Eschenfeldt, Cynthia Sanville Millard, James Abdullah, Frank R Collart, Youngchang Kim, Mark I Donnelly
New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols.
Protein Expr Purif: 2007, 53(2);396-403
[PubMed:17363272] [WorldCat.org] [DOI] (P p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)