Rnc

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  • Description: RNase III

Gene name rnc
Synonyms rncS
Essential yes
Product endoribonuclease III
Function cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
MW, pI 28 kDa, 8.076
Gene length, protein length 747 bp, 249 aa
Immediate neighbours acpA, smc
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Rnc context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15930

Phenotypes of a mutant

essential PubMed

rnc is essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number: 3.1.26.3

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References

Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666] [WorldCat.org] [DOI] (I p)

M A Herskovitz, D H Bechhofer
Endoribonuclease RNase III is essential in Bacillus subtilis.
Mol Microbiol: 2000, 38(5);1027-33
[PubMed:11123676] [WorldCat.org] [DOI] (P p)

A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377] [WorldCat.org] [DOI] (P p)

W Wang, D H Bechhofer
Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci.
J Bacteriol: 1997, 179(23);7379-85
[PubMed:9393702] [WorldCat.org] [DOI] (P p)

S Mitra, D H Bechhofer
Substrate specificity of an RNase III-like activity from Bacillus subtilis.
J Biol Chem: 1994, 269(50);31450-6
[PubMed:7527387] [WorldCat.org] (P p)