rapK

168

response regulator aspartate phosphatase, controls ComA activity

Locus: BSU_18910

Molecular weight: 43.68 kDa

Isoelectric point: 5.06

Protein length: 371 aa

Gene length: 1116 bp

Function: control of ComA activity

Product: response regulator aspartate phosphatase

Essential: no

Synonyms: rapK, yobG

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,062,150 → 2,063,265

The protein

Catalyzed reaction/ biological activity

• control of ComA activity PubMed

Protein family

• RAP family (according to UniProt)

Domains

• six tetratrichopeptide repeats (according to UniProt)

Structure

• 4I1A (PDB) (RapI, 30% identity) PubMed
• O34930 (AlphaFold)

Effectors of protein activity

• binding of PhrK inhibits RapK activity PubMed

Expression and Regulation

Operons

• Genes: rapK-phrK

  Description: PubMed

  Regulation

  • induced under conditions of phosphate limitation (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PubMed, in phoP regulon
  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• BKE18910 (ΔrapK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAAACCCCTCTTTCTG,  downstream forward: _UP4_ATGAATCAGGTGGAGGGAAT
• BKK18910 (ΔrapK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAAACCCCTCTTTCTG,  downstream forward: _UP4_ATGAATCAGGTGGAGGGAAT

References

Reviews

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Original Publications

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