pspA

168

phage shock protein A homolog, protects the membrane against membrane-targeting antibiotics

Locus: BSU_06180

Molecular weight: 24.99 kDa

Isoelectric point: 5.78

Protein length: 227 aa

Gene length: 684 bp

Function: protection of the membrane

Product: phage shock protein A homolog

Essential: no

Synonyms: pspA, ydjF

Genomic Context

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List of homologs in different organisms, belongs to COG1842 (Galperin et al., 2021)

Gene

Coordinates: 671,245  671,928

Phenotypes of a mutant

• more sensitive to nisin PubMed

The protein

Protein family

• PspA/IM30 family (with LiaH, according to UniProt)

Structure

• 4WHE (PDB) (from E. coli, 34% identity, corresponds to aa 1 ... 133) PubMed
• P54617 (AlphaFold)

Paralogous protein(s)

• LiaH

Localization

• cytoplasma (according to UniProt)
• localizes to the membrane under stress conditions (localization depends of YdjI) PubMed

Expression and Regulation

Operons

• Genes: pspA-ydjG-ydjH-ydjI

  Description: PubMed

  Regulation

  • induced by alkaline shock (SigW) PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• MGNA-C430 (yqgI::erm), available at the NBRP B. subtilis, Japan
• BKE06180 (pspA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATATCGTTCCTCCTAA,  downstream forward: _UP4_TAATACATAGGAGGCCGCAG
• BKK06180 (pspA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATATCGTTCCTCCTAA,  downstream forward: _UP4_TAATACATAGGAGGCCGCAG

References

Reviews

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Original Publications

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