ppsA

168

plipastatin synthetase

Locus: BSU_18340

Molecular weight: 288.86 kDa

Isoelectric point: 5.22

Protein length: 2561 aa

Gene length: 7686 bp

Function: production of the antibacterial compound plipastatin

Product: plipastatin synthetase

Essential: no

Synonyms: ppsA

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Metabolism, Additional metabolic pathways, Miscellaneous metabolic pathways, Biosynthesis of antibacterial compounds

Lifestyles, Coping with stress, Biosynthesis of antibacterial compounds

List of homologs in different organisms, belongs to COG4908 (Galperin et al., 2021)

This gene is a member of the following regulons

AbrB regulon

Gene

Coordinates: 1,990,272 → 1,997,957

The protein

Protein family

ATP-dependent AMP-binding enzyme family (according to UniProt)

Domains

2 Carrier domains (aa 961-1036, aa 2007-2081) (according to UniProt)

Structure

2VSQ (PDB) (termination module of SrfAC, 30% identity) PubMed
P39845 (AlphaFold)

Expression and Regulation

Operons

Genes: ppsA-ppsB-ppsC-ppsD-ppsE

Description: PubMed

Regulatory mechanism

AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

BKE18340 (ΔppsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACGGATTCCCTCCAGTTC,  downstream forward: _UP4_TAACTTTTGAAAAGGTGTGT
BKK18340 (ΔppsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACGGATTCCCTCCAGTTC,  downstream forward: _UP4_TAACTTTTGAAAAGGTGTGT

References

Loading