pksG

168

involved in polyketide synthesis

Locus: BSU_17150

Molecular weight: 46.62 kDa

Isoelectric point: 5.14

Protein length: 420 aa

Gene length: 1263 bp

Function: polyketide synthesis

Product: hydroxymethylglutaryl CoA synthase

Essential: no

Synonyms: pksG

Genomic Context

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List of homologs in different organisms, belongs to COG3425 (Galperin et al., 2021)

Gene

Coordinates: 1,789,943 → 1,791,205

The protein

Catalyzed reaction/ biological activity

• 3-oxobutanoyl-[ACP] + acetyl-[ACP] + H₂O --> (3S)-hydroxy-3-methylglutaryl-[ACP] + H⁺ + holo-[ACP] (according to UniProt)

Protein family

• thiolase-like superfamily (according to UniProt)

Structure

• 4YXQ (PDB)
• 4YXT (PDB) (E82A mutant)
• 4YXV (PDB) (C114A mutant)
• P40830 (AlphaFold)

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed
  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • CodY: activation, PubMed, in codY regulon

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

Biological materials

Mutant

• MGNA-A017 (pksG::erm), available at the NBRP B. subtilis, Japan
• BKE17150 (ΔpksG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA,  downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA
• BKK17150 (ΔpksG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA,  downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA

References

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