pghL

168

phage-derived gamma polyglutamic acid hydrolase, sporulation protein

Locus: BSU_17820

Molecular weight: 28.07 kDa

Isoelectric point: 6.92

Protein length: 252 aa

Gene length: 759 bp

Function: polyglutamic acid degradation

Product: phage-derived gamma polyglutamic acid hydrolase

Essential: no

Synonyms: pghL, yndL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4195 (Galperin et al., 2021)

Gene

Coordinates: 1,915,221 → 1,915,979

The protein

Catalyzed reaction/ biological activity

• hydrolysis of polyglutamic acid PubMed

Protein family

• UPF0714 family (according to UniProt)

Domains

• DUF867 x, [[gamma-PGA hydrolase domain]] PubMed

Structure

• 5ONJ (PDB) (complex with poly-γ-glutamate) PubMed
• 3A9L (PDB) (from B. subtilis phage NIT1, 36% identity) PubMed
• 6HRI (PDB) (native)
• 6HRJ (PDB) (apo-form)
• O31815 (AlphaFold)

Paralogous protein(s)

• PghZ, PghB, PghC

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: pghL

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cellPubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • An [antisense RNA], 'surA', is predicted for 'pghL' PubMed

Biological materials

Mutant

• MGNA-B113 (yndL::erm), available at the NBRP B. subtilis, Japan
• BKE17820 (ΔpghL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT,  downstream forward: _UP4_TGAAAAATAGGAGGCGATAA
• BKK17820 (ΔpghL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTACTCCGCCTCTAAGT,  downstream forward: _UP4_TGAAAAATAGGAGGCGATAA

References

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