paiA

168

spermine/spermidine-N-acetyltransferase

Locus: BSU_32150

Molecular weight: 19.87 kDa

Isoelectric point: 5.02

Protein length: 172 aa

Gene length: 519 bp

Function: control of intracellular polyamine concentrations

Product: spermine/spermidine-N-acetyltransferase

Essential: no

Synonyms: paiA

Genomic Context

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List of homologs in different organisms, belongs to COG0456 (Galperin et al., 2021)

Gene

Coordinates: 3,304,743  3,305,261

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + alkane-α,ω-diamine --> N-acetylalkane-α,ω-diamine + CoA + H⁺ (according to UniProt)

Domains

• N-acetyltransferase domain (aa 3-172) (according to UniProt)

Structure

• 1TIQ (PDB)
• P21340 (AlphaFold)

Expression and Regulation

Operons

• Genes: paiA-paiB

  Description: PubMed

Biological materials

Mutant

• BKE32150 (paiA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTCGGATTCAGCCCC,  downstream forward: _UP4_TAATATTTTTCGAAGGGTGG
• BKK32150 (paiA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTCGGATTCAGCCCC,  downstream forward: _UP4_TAATATTTTTCGAAGGGTGG

References

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