mtbP

168

DNA (cytosine-5-)-methyltransferase

Locus: BSU_20250

Molecular weight: 50.35 kDa

Isoelectric point: 7.8

Protein length: 443 aa

Gene length: 1332 bp

Function: DNA modification

Product: DNA (cytosine-5-)-methyltransferase

Essential: no

E.C.: 2.1.1.37

Synonyms: mtbP

Genomic Context

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List of homologs in different organisms, belongs to COG0270 (Galperin et al., 2021)

Gene

Coordinates: 2,171,401 → 2,172,732

The protein

Catalyzed reaction/ biological activity

• 2'-deoxycytidine in DNA + S-adenosyl-L-methionine --> 5-methyl-2'-deoxycytidine in DNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Domains

• SAM-dependent MTase C5-type domain (aa 4-440) (according to UniProt)

Structure

• 1MHT (PDB) (from Haemophilus haemolyticus, 28% identity) PubMed
• P68585 (AlphaFold)

Biological materials

Mutant

• BKE20250 (ΔmtbP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAAACAACCTCCATATA,  downstream forward: _UP4_TAAAATTTGTCTTTTAAACA
• BKK20250 (ΔmtbP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAAACAACCTCCATATA,  downstream forward: _UP4_TAAAATTTGTCTTTTAAACA

References

Research papers

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