tagU

168

phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

Locus: BSU_35650

Molecular weight: 34.43 kDa

Isoelectric point: 9.53

Protein length: 306 aa

Gene length: 921 bp

Function: transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan

Product: phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan

Essential: no

Synonyms: tagU, lytR

Genomic Context

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List of homologs in different organisms, belongs to COG1316 (Galperin et al., 2021)

Gene

Coordinates: 3,663,281  3,664,201

Phenotypes of a mutant

• a ''tagU mutant has no phenoype, the triple tagT tagU tagV'' mutant is unable to grow under normal conditions PubMed

The protein

Protein family

• LytR/CpsA/Psr (LCP) family (with TagV and TagT, according to UniProt)  PubMed

Cofactors

• Mg²⁺ PubMed

Structure

• 6UF6 (PDB) (aa 62-306) PubMed
• 3MEJ (PDB) (TagT, 36% identity, 69% similarity)
• Q02115 (AlphaFold)

Paralogous protein(s)

• TagT, TagV

Localization

• cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed

Expression and Regulation

Operons

• Genes: tagU

  Description: PubMed

  Regulatory mechanism

  • TagU: auto-repression, PubMed, in tagU regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigM: sigma factor, PubMed, in sigM regulon
  • SigX: sigma factor, PubMed, in sigX regulon

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• BKE35650 (tagU::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTTTGCACCTCGTCTG,  downstream forward: _UP4_TAAAACAAAAAGAAGCTTCG
• BKK35650 (tagU::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTTTGCACCTCGTCTG,  downstream forward: _UP4_TAAAACAAAAAGAAGCTTCG

References

Reviews

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Original Publications

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