LiaS

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  • Description: two-component sensor kinase, response to bacitracin

Gene name liaS
Synonyms yvqE
Essential no
Product two-component sensor kinase (NarL family)
Function control of LiaR activity in response to bacitracin
MW, pI 40 kDa, 7.333
Gene length, protein length 1080 bp, 360 aa
Immediate neighbours liaR, liaF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvqE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU33090

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

liaS-liaR

  • Regulation: liaI: induced by bacitracin (LiaR)

liaS: constitutive

  • Regulatory mechanism: LiaR: transcription activation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Thorsten Mascher, Karlsruhe Institute of Technology, Germany link

Your additional remarks

References

Sina Jordan, Anja Junker, John D Helmann, Thorsten Mascher
Regulation of LiaRS-dependent gene expression in bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system.
J Bacteriol: 2006, 188(14);5153-66
[PubMed:16816187] [WorldCat.org] [DOI] (P p)

  1. Jordan, S., A. Junker, J. D. Helmann, and T. Mascher. 2006. Regulation of LiaRS-dependent gene expression in Bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system. J. Bacteriol. 188: 5153-5166. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed