Difference between revisions of "KsgA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || rRNA maturation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || rRNA maturation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00420 ksgA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00420 ksgA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnmV]]'', ''[[yabG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnmV]]'', ''[[yabG]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11818&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00420 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00420 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00420 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ksgA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ksgA_context.gif]]

Revision as of 11:54, 13 May 2013

  • Description: rRNA adenine dimethyltransferase

Gene name ksgA
Synonyms
Essential no
Product rRNA adenine dimethyltransferase
Function rRNA maturation
Gene expression levels in SubtiExpress: ksgA
MW, pI 32 kDa, 5.712
Gene length, protein length 876 bp, 292 aa
Immediate neighbours rnmV, yabG
Sequences Protein DNA Advanced_DNA
Genetic context
KsgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
KsgA expression.png
























Categories containing this gene/protein

translation, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU00420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: methylation of A1518 and A1519 in 16S rRNA
  • Protein family: KsgA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kozo Ochi, Ji-Yun Kim, Yukinori Tanaka, Guojun Wang, Kenta Masuda, Hideaki Nanamiya, Susumu Okamoto, Shinji Tokuyama, Yoshikazu Adachi, Fujio Kawamura
Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance.
Antimicrob Agents Chemother: 2009, 53(1);193-201
[PubMed:19001112] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

C Condon, D Brechemier-Baey, B Beltchev, M Grunberg-Manago, H Putzer
Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function.
RNA: 2001, 7(2);242-53
[PubMed:11233981] [WorldCat.org] [DOI] (P p)