DefB

• Description: major formylmethionine deformylase
  
  

Gene name	ykrB
Synonyms	defB
Essential	no
Product	formylmethionine deformylase
Function	translation
Gene expression levels in SubtiExpress: ykrB
MW, pI	20 kDa, 5.456
Gene length, protein length	552 bp, 184 aa
Immediate neighbours	ykrA, ykzV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, protein modification

The gene

Basic information

• Locus tag: BSU14560

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Formyl-L-methionyl peptide + H₂O = formate + methionyl peptide (according to Swiss-Prot)

• Protein family: polypeptide deformylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)

• UniProt: Q45495

• KEGG entry: [2]

• E.C. number: 3.5.1.88

Expression and regulation

• Operon:

• Expression browser: ykrB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)