DeaD

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Revision as of 15:09, 5 July 2012 by Jstuelk (talk | contribs) (Biological materials)
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Gene name deaD
Synonyms yxiN
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
Interactions involving this protein in SubtInteract: DeaD
MW, pI 53 kDa, 7.706
Gene length, protein length 1437 bp, 479 aa
Immediate neighbours yxiO, yxiM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DeaD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DeaD expression.png
























Categories containing this gene/protein

DEAD-box RNA helicases, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU39110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Helicase domain 1: AS 1-203
    • Linker region: AS 204-212
    • Helicase domain 2: AS 207-368
    • RNA-binding domain: AS 404-479
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2G0C (RNA-binding domain, AA 404-479), 2HJV (second domain, AA 207-368); 3MOJ (RNA binding domain complexed with a fragment of 23S ribosomal RNA) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1052 (tet), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct: GP1065 (spc, based on pGP1331), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

Your additional remarks

References