dacA

168

Class C penicillin-binding protein 5, major D-alanyl-D-alanine carboxypeptidase

Locus: BSU_00100

Molecular weight: 48.47 kDa

Isoelectric point: 5.67

Protein length: 443 aa

Gene length: 1332 bp

Function: carboxypeptidase

Product: penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase

Essential: no

Synonyms: dacA

Genomic Context

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List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)

Gene

Coordinates: 17,534  18,865

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• the mutant cells are thicker and shorter than wild type cells PubMed
• sensitivity to most β-lactams, and strong resistance to vancomycin PubMed
• defective in swarming motility (reduced rate of colony expansion) PubMed

The protein

Catalyzed reaction/ biological activity

• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to UniProt)

Protein family

• Peptidase S11 family (according to UniProt)

Structure

• 1XP4 (PDB) (from Streptococcus pneumoniae, 37% identity) PubMed
• P08750 (AlphaFold)

Paralogous protein(s)

• DacF

Localization

• secreted (according to Swiss-Prot)
• membrane associated PubMed
• during vegetative growth: septal, distinct spots at periphery PubMed

Expression and Regulation

Operons

• Genes: dacA-pdxS-pdxT-serS

  Description: PubMed

  Regulation

  • the leader mRNA is processed upstream of dacA by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Biological materials

Mutant

• 1A742 ( dacA::cat), PubMed, available at BGSC
• 1A743 ( dacA::cat), PubMed, available at BGSC
• BKE00100 (dacA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCGTACGACCTCCGTAT,  downstream forward: _UP4_TAATCAATTGAAAGAGCTCT
• BKK00100 (dacA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCGTACGACCTCCGTAT,  downstream forward: _UP4_TAATCAATTGAAAGAGCTCT

GFP fusion

• 2085 trpC2 dacA::pSG1493 (cat P_xyl-gfpa-dacA1-423) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

References

Reviews

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Original Publications

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