ccpB

168

transcriptional regulator (LacI family)

Locus: BSU_40870

Molecular weight: 34.69 kDa

Isoelectric point: 6.33

Protein length: 311 aa

Gene length: 936 bp

Function: unknown

Product: transcriptional regulator (LacI family)

Essential: no

Synonyms: ccpB, yyaG

Genomic Context

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List of homologs in different organisms, belongs to COG1609 (Galperin et al., 2021)

Gene

Coordinates: 4,196,786 → 4,197,721

The protein

Protein family

• LacI family

Domains

• HTH lacI-type domain (aa 1-56) (according to UniProt)

Structure

• 2O20 (PDB) (Lactococcus lactis, 27.9% identity) PubMed
• P37517 (AlphaFold)

Expression and Regulation

Operons

• Genes: exoA-ccpB

  Description: (according to DBTBS) null

  Regulation

  • expressed late during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-B867 (yyaG::erm), available at the NBRP B. subtilis, Japan
• BKE40870 (ΔccpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTTTCTCCTTTTCAT,  downstream forward: _UP4_TGAACTGAAATGCATTTCAT
• BKK40870 (ΔccpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTTTCTCCTTTTCAT,  downstream forward: _UP4_TGAACTGAAATGCATTTCAT

References

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