Difference between revisions of "BacG"
| Line 4: | Line 4: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''bacG'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-86r '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-86r, ywfH '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the antibiotic bacilysin | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of the antibiotic bacilysin | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37680 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37680 bacG] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 6.596 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 6.596 | ||
| Line 20: | Line 20: | ||
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 777 bp, 259 aa | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hemQ]]'', ''[[bacF]]'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15795]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15795]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | ||
| Line 68: | Line 68: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| − | ** | + | ** BacG catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate {{PubMed|20052993}} |
** stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP {{PubMed|23519407}} | ** stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP {{PubMed|23519407}} | ||
| Line 83: | Line 83: | ||
* '''Modification:''' | * '''Modification:''' | ||
| − | * '''Cofactor(s):''' NADPH | + | * '''Cofactor(s):''' NADPH {{PubMed|23519407}} |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 105: | Line 105: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' '' | + | * '''Operon:''' ''bacG'' {{PubMed|12372825}} |
| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywfH_3867493_3868272_1 bacG] {{PubMed|22383849}} |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
Revision as of 14:37, 27 March 2013
- Description: short chain reductase involved in bacilysin synthesis
| Gene name | bacG |
| Synonyms | ipa-86r, ywfH |
| Essential | no |
| Product | short chain reductase |
| Function | biosynthesis of the antibiotic bacilysin |
| Gene expression levels in SubtiExpress: bacG | |
| MW, pI | 27 kDa, 6.596 |
| Gene length, protein length | 777 bp, 259 aa |
| Immediate neighbours | hemQ, bacF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, biosynthesis of antibacterial compounds
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37680
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- BacG catalyzes the conjugate addition of hydride at the C4 olefinic terminus using NADH to yield the cyclohexenol- containing tetrahydro-4-hydroxyphenylpyruvate PubMed
- stereoselective reduction of dihydro-hydroxyphenylpyruvate (H2HPP) to tetrahydro-hydroxyphenylpyruvate (H4HPP), NADPH-dependent reductase that facilitates the conjugate addition of a hydride at the C(4) olefin terminus of H2HPP PubMed
- Protein family: short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): NADPH PubMed
- Effectors of protein activity:
Database entries
- UniProt: P39644
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon: bacG PubMed
- Regulation:
- repressed by casamino acids PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Malligarjunan Rajavel, Kumar Perinbam, B Gopal
Structural insights into the role of Bacillus subtilis YwfH (BacG) in tetrahydrotyrosine synthesis.
Acta Crystallogr D Biol Crystallogr: 2013, 69(Pt 3);324-32
[PubMed:23519407]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kozo Ochi
Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(22);8181-3
[PubMed:21948839]
[WorldCat.org]
[DOI]
(I p)
Sarah A Mahlstedt, Christopher T Walsh
Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Biochemistry: 2010, 49(5);912-23
[PubMed:20052993]
[WorldCat.org]
[DOI]
(I p)
Takashi Inaoka, Kosaku Takahashi, Mayumi Ohnishi-Kameyama, Mitsuru Yoshida, Kozo Ochi
Guanine nucleotides guanosine 5'-diphosphate 3'-diphosphate and GTP co-operatively regulate the production of an antibiotic bacilysin in Bacillus subtilis.
J Biol Chem: 2003, 278(4);2169-76
[PubMed:12372825]
[WorldCat.org]
[DOI]
(P p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)
