bacB

168

oxidase that catalyzes the synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin

Locus: BSU_37730

Molecular weight: 26.69 kDa

Isoelectric point: 5.19

Protein length: 235 aa

Gene length: 708 bp

Function: biosynthesis of the antibiotic bacilysin

Product: oxidase

Essential: no

Synonyms: bacB, ywfC, ipa-81d

Genomic Context

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List of homologs in different organisms, belongs to COG1917 (Galperin et al., 2021)

Gene

Coordinates: 3,872,869  3,873,576

The protein

Catalyzed reaction/ biological activity

• synthesis of 2-oxo-3-(4-oxocyclohexa-2,5-dienyl)propanoic acid, a precursor to L-anticapsin PubMed
• 3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate --> 3-[(1E,4R)-4-hydroxycyclohex-2-en-1-ylidene]pyruvate (according to UniProt)

Domains

• 2 cupin 2 domains (aa 41 ... 106, aa 151 ... 216)

Structure

• 3H7J (PDB) PubMed
• P39639 (AlphaFold)

Expression and Regulation

Operons

• Genes: bacA-bacB-bacC-bacD-bacE-bacF

  Description: PubMed

  Regulation

  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon
  • AbrB: repression, PubMed, in abrB regulon
  • ScoC: repression, PubMed, in scoC regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B240 (ywfC::erm), available at the NBRP B. subtilis, Japan
• BKE37730 (bacB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAAATAAAGCTCCTGCATAT,  downstream forward: _UP4_AAAATGAAGGCGGATGAATG
• BKK37730 (bacB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAAATAAAGCTCCTGCATAT,  downstream forward: _UP4_AAAATGAAGGCGGATGAATG

References

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