metS

168

methionyl-tRNA synthetase

Locus: BSU_00380

Molecular weight: 76.01 kDa

Isoelectric point: 4.96

Protein length: 664 aa

Gene length: 1995 bp

Function: translation

Product: methionyl-tRNA synthetase

Essential: yes

E.C.: 6.1.1.10

Synonyms: metS, metG

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Information processing, Protein synthesis, modification and degradation, Translation, Aminoacyl-tRNA synthetases

Groups of genes, Essential genes

Groups of genes, Phosphoproteins, Phosphorylation on a Ser residue

List of homologs in different organisms, belongs to COG0143 (Galperin et al., 2021)

Gene

Coordinates: 45,633 → 47,627

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

ATP + L-methionine + tRNA^Met --> AMP + diphosphate + L-methionyl-tRNA^Met (according to UniProt)

Protein family

Class-I aminoacyl-tRNA synthetase family (according to UniProt)

Domains

tRNA-binding domain (aa 570-662) (accordinig to UniProt)

Structure

4QRD (PDB) (from Staphylococcus aureus, 67.9% identity)
1WE0 (PDB) (from Thermus thermophilus hb8 mutant, 40% identity, 57% similarity) PubMed
P37465 (AlphaFold)

Modification

phosphorylated on Ser-652 PubMed

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: metS-yabD

Description: PubMed null

Regulation

repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism

Spo0A: repression, PubMed, in spo0A regulon

Additional information

term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of metS PubMed

Genes: metS

Description: null

Regulation

repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism

Spo0A: repression, PubMed, in spo0A regulon

Additional information

term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of metS PubMed

References

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