pstA

168

phosphate ABC transporter (permease)

Locus: BSU_24970

Molecular weight: 31.31 kDa

Isoelectric point: 9.3

Protein length: 294 aa

Gene length: 885 bp

Function: high-affinity phosphate uptake

Product: phosphate ABC transporter (permease)

Essential: no

Synonyms: pstA, yqgI, yzmD

Genomic Context

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List of homologs in different organisms, belongs to COG0581 (Galperin et al., 2021)

Gene

Coordinates: 2,578,833  2,579,717

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• CysTW subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 62-280) (according to UniProt)

Structure

• P46340 (AlphaFold)

Paralogous protein(s)

• PstC

Localization

• membrane protein PubMed

Expression and Regulation

Operons

• Genes: pstS-pstC-pstA-pstBA-pstBB

  Description: PubMed

  Regulation

  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PhoP-P, PubMed, in phoP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed

Biological materials

Mutant

• BKE24970 (pstA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTATCTGTTATTTTGCGGT,  downstream forward: _UP4_TAGAACGGAAAGAGGCGAAA
• BKK24970 (pstA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTATCTGTTATTTTGCGGT,  downstream forward: _UP4_TAGAACGGAAAGAGGCGAAA

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

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