cotO

168

spore coat morphogenetic protein, controls assembly of the coat layers and coat surface topography

Locus: BSU_11730

Molecular weight: 25.45 kDa

Isoelectric point: 6.38

Protein length: 227 aa

Gene length: 684 bp

Function: controls assembly of the coat layers and coat surface topography

Product: spore coat morphogenetic protein

Essential: no

Synonyms: cotO, yjbX

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5885 (Galperin et al., 2021)

Gene

Coordinates: 1,248,665  1,249,348

Phenotypes of a mutant

• spores are defective in germination PubMed

The protein

Catalyzed reaction/ biological activity

• necessary for proper encasement of the spore by the crust PubMed

Structure

• O31622 (AlphaFold)

Localization

• outer spore coat, localization depends on CotE PubMed

Expression and Regulation

Operons

• Genes: cotO

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B169 (yjbX::erm), available at the NBRP B. subtilis, Japan
• BKE11730 (cotO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCTCCGATTGTGTCAT,  downstream forward: _UP4_TAACGAAAAAGAAAAAAGCT
• BKK11730 (cotO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCTCCGATTGTGTCAT,  downstream forward: _UP4_TAACGAAAAAGAAAAAAGCT

References

Reviews

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Original Publications

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