btr

168

transcriptional activator ([wiki|AraC family]), (AraC family DNA-binding domain fused to [protein|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-like substrate-binding domain), regulation of the [gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA] operon

locus: BSU_01640

Molecular weight: 60.59 kDa

pI: 6.43

Protein length: 529 aa

Gene length: 1590 bp

function: regulation of iron acquisition

product: transcription activator ([wiki|AraC family])

essential: no

synonyms: btr, ybbB, yzbC

List of homologs in different organisms, belongs to COG2207 (Galperin et al., 2021)

Gene

Coordinates: 183,414 → 185,003

The protein

Catalyzed reaction/ biological activity

• binding of a direct repeat in the [gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA] promoter region and transcription activation of the [gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA] operon in the presence of bacillibactin or enterobactin [Pubmed|17725565,22210890]

Protein family

• [wiki|AraC family]

[wiki|Domains]

• [wiki|Fe/B12 periplasmic-binding domain] (aa 268-528) (according to UniProt)

[wiki|Cofactors]

• enterobactin, bacillibactin (co-activators) [Pubmed|17725565]

Structure

• [AF|P40408]

Effectors of protein activity

• enterobactin, bacillibactin (co-activators) [Pubmed|17725565]

Expression and Regulation

Operons

• genes: [gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]

  description: [Pubmed|12354229]

  regulation

  • expressed under conditions of iron starvation ([protein|search|Fur]) [Pubmed|12354229]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-B949 (ybbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1948 NBRP B. subtilis, Japan]
• BKE01640 (Δ[gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGAGTAGACCACCTTTTC,  downstream forward: _UP4_TGATAAATCAGGAATTTGTC
• BKK01640 (Δ[gene|EB28A65CECE994DF2DF486DEACF40F2533703DB0|btr]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGAGTAGACCACCTTTTC,  downstream forward: _UP4_TGATAAATCAGGAATTTGTC

References

• 12884008,17725565,22210890,12354229,23199363,23504016