QueF

• Description: nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine
  
  

Gene name	queF
Synonyms	ykvM
Essential	no
Product	nitrile reductase
Function	tRNA modification
Gene expression levels in SubtiExpress: queF
MW, pI	19 kDa, 4.927
Gene length, protein length	495 bp, 165 aa
Immediate neighbours	queE, ykvN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

preQ1 riboswitch

The gene

Basic information

• Locus tag: BSU13750

Database entries

• BsubCyc: BSU13750

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH (according to Swiss-Prot)

• Protein family: QueF type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: active site Cys56 is S-bacillithiolated by NaOCl stress PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13750

• Structure: 4F8B (covalent thioimide intermediate of the unimodular nitrile reductase QueF) PubMed

• UniProt: O31678

• KEGG entry: [2]

• E.C. number: 1.7.1.13

Expression and regulation

• Operon: queC-queD-queE-queF PubMed

• Expression browser: queF PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed

• Regulatory mechanism:
  • preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 303 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 913 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: