yxeN

168

putative S-(2-succino)cysteine ABC transporter (permease)

Locus: BSU_39490

Molecular weight: 24.86 kDa

Isoelectric point: 9.48

Protein length: 224 aa

Gene length: 675 bp

Function: uptake and utilization of S-(2-succino)cysteine

Product: putative S-(2-succino)cysteine ABC transporter (permease)

Essential: no

Synonyms: yxeN

Genomic Context

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List of homologs in different organisms, belongs to COG0765 (Galperin et al., 2021)

Gene

Coordinates: 4,058,791 → 4,059,465

The protein

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 20-211) (according to UniProt)

Structure

• 4YMS (PDB) (amino acid transporter from Caldanaerobacter subterraneus, 39% identity) PubMed
• P54953 (AlphaFold)

Paralogous protein(s)

• TcyL, TcyB, YckA

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yxeK-snaB-yxeM-yxeN-yxeO-sndB-yxeQ

  Description: PubMed

  Regulation

  • repressed in the presence of cysteine (CymR) PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

Mutant

• GP4477 (trpC2 ΔyxeN::neo), available in Jörg Stülke's lab
• GP4677 (trpC2 ΔyxeN::lox72), available in Jörg Stülke's lab
• MGNA-B710 (yxeN::erm), available at the NBRP B. subtilis, Japan
• 1A950 ( yxeN::cat), PubMed, available at BGSC
• BKE39490 (ΔyxeN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC,  downstream forward: _UP4_TACCGGACTTAGAGGTGAAA
• BKK39490 (ΔyxeN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTGGTGAGTCACCGCC,  downstream forward: _UP4_TACCGGACTTAGAGGTGAAA

References

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