spsI

168

glucose-1-phosphate thymidylyltransferase

Locus: BSU_37840

Molecular weight: 27.62 kDa

Isoelectric point: 5.39

Protein length: 246 aa

Gene length: 741 bp

Function: rhamnose biosynthesis, spore crust polysaccharide synthesis

Product: glucose-1-phosphate thymidylyltransferase

Essential: no

E.C.: 2.7.7.24

Synonyms: spsI, ipa-71d

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1209 (Galperin et al., 2021)

Gene

Coordinates: 3,885,239  3,885,979

Phenotypes of a mutant

• production of spores lacking a visible crust PubMed

The protein

Catalyzed reaction/ biological activity

• α-D-glucose-1-phosphate + dTTP + H⁺ --> dTDP-α-D-glucose + pp_i PubMed

Protein family

• glucose-1-phosphate thymidylyltransferase family (single member, according to UniProt)

Structure

• 3HL3 (PDB) (from B. anthracis, 55% identity)
• P39629 (AlphaFold)

Localization

• outer spore coat PubMed

Expression and Regulation

Operons

• Genes: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE37840 (spsI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATTAACACTTCCTTACA,  downstream forward: _UP4_CGGAAAGGACAGGACGAAAA
• BKK37840 (spsI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATTAACACTTCCTTACA,  downstream forward: _UP4_CGGAAAGGACAGGACGAAAA

References

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