ytpA

168

phospholipase (annotated as a bacilysocin synthase)

Locus: BSU_30510

Molecular weight: 29.42 kDa

Isoelectric point: 6.77

Protein length: 259 aa

Gene length: 780 bp

Function: lipid degradation

Product: phospholipase

Essential: no

Synonyms: ytpA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2267 (Galperin et al., 2021)

Gene

Coordinates: 3,122,662  3,123,441

The protein

Catalyzed reaction/ biological activity

• cleaves fatty acids from the 2 position of phosphatidylglycerol PubMed

Protein family

• AB hydrolase superfamily (according to UniProt)

Domains

• 3 AB hydrolase-1 domains (aa 37-52, aa 84-97, aa 198-212) (according to InterPro)

Structure

• 3HJU (PDB) (human monoglyceride lipase, 30% identity) PubMed
• O34705 (AlphaFold)

Expression and Regulation

Operons

• Genes: ytpA-ytpB

  Description: PubMed

  Regulation

  • expressed under conditions of salt stress (SigM) PubMed
  • induced by cell wall-inhibiting drugs, such as bacitracin and cefuroxime (SigM) PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon

Biological materials

Mutant

• MGNA-A128 (ytpA::erm), available at the NBRP B. subtilis, Japan
• BKE30510 (ytpA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCTTCACCTCATACG,  downstream forward: _UP4_TGAACTAGTAGGGGGTGCAT
• BKK30510 (ytpA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATCTTCACCTCATACG,  downstream forward: _UP4_TGAACTAGTAGGGGGTGCAT

References

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