infC

168

translation initiation factor IF-3, prevents premature docking of 50S ribosomal subunits

Locus: BSU_28870

Molecular weight: 19.59 kDa

Isoelectric point: 10.34

Protein length: 173 aa

Gene length: 522 bp

Function: translation

Product: initiation factor IF-3

Essential: yes

Synonyms: infC

Genomic Context

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List of homologs in different organisms, belongs to COG0290 (Galperin et al., 2021)

Gene

Coordinates: 2,952,828 → 2,953,349

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• prevents premature docking of 50S ribosomal subunits PubMed

Protein family

• IF-3 family (single member, according to UniProt)

Structure

• 1TIF (PDB) (N-terminal domain, Geobacillus stearothermophilus),  1TIG (PDB) (C-terminal domain, Geobacillus stearothermophilus)
• P55872 (AlphaFold)

Expression and Regulation

Operons

• Genes: infC-rpmI-rplT-ysdA

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • RplT: termination, via binding to a RNA switch in the infC leader region causes transcription termination, in rplT regulon

  Additional information

  • autorepression of infC-rpmI-rplT-ysdA expression upon binding of excess RplT to the untranslated region of the mRNA PubMed
  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent mRNA processing PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

References

Reviews

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Original Publications

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