leuA

168

2-isopropylmalate synthase

Locus: BSU_28280

Molecular weight: 56.74 kDa

Isoelectric point: 5.66

Protein length: 518 aa

Gene length: 1557 bp

Function: biosynthesis of leucine

Product: 2-isopropylmalate synthase

Essential: no

E.C.: 2.3.3.13

Synonyms: leuA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0119 (Galperin et al., 2021)

Gene

Coordinates: 2,892,138 2,893,694

The protein

Catalyzed reaction/ biological activity

• 3-methyl-2-oxobutanoate + acetyl-CoA + H₂O --> (2S)-2-isopropylmalate + CoA + H⁺ (according to UniProt)

Protein family

• alpha-IPM synthase/homocitrate synthase family (single member, according to UniProt)

Domains

• Pyruvate carboxyltransferase domain (aa 4-266) (according to UniProt)

Structure

• 3EEG (PDB) (from Cytophaga hutchinsonii atcc 33406, 53% identity, 68% similarity)
• P94565 (AlphaFold)

Localization

• cytoplasm (according to UniProt)
• membrane PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD

  Description: PubMed

  Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed

  Regulatory mechanism

  • T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in tnrA regulon
  • CodY: repression, PubMed PubMed, in codY regulon
  • CcpA: activation, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• BKE28280 (leuA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT
• BKK28280 (leuA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT

References

Loading