pbpI

168

[wiki|sporulation]-specific class B penicillin-binding protein 4b

locus: BSU_27310

Molecular weight: 65.35 kDa

pI: 9.01

Protein length: 584 aa

Gene length: 1755 bp

function: unknown

product: penicillin-binding protein 4b

essential: no

synonyms: pbpI, yrrR, pbpI

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

Gene

Coordinates: 2,789,686  2,791,440

The protein

Protein family

• [wiki|transpeptidase family] (according to UniProt)

Structure

• [PDB|3OC2] (PBP3 from Pseudomonas aeruginosa, 23% identity) [pubmed|20974151]
• [AF|O32032]

[wiki|Localization]

• anchored to the membrane, the major part is exposed to the outside (according to UniProt)
• during vegetative growth: evenly distributed along the membrane [pubmed|14731276]

Expression and Regulation

Operons

• genes: [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]-[gene|9892E5BFE90EC934A4047795BC4B328656128C09|yrrS]

  description: [Pubmed|22383849]

  regulation

  • expressed early during sporulation in both cell compartments ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE] [Pubmed|14679248,15758244], [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF] [Pubmed|15699190,16497325])

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|14679248,15758244], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|15699190,16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• BKE27310 ([gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC,  downstream forward: _UP4_TAGAACAACTTCTCAGCACA
• BKK27310 ([gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC,  downstream forward: _UP4_TAGAACAACTTCTCAGCACA

GFP fusion

• 2081 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::pSG1489 (cat P_xyl-gfpa-[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]1-430) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

Reviews

• 33709487

Original Publications

• 14679248,15699190,16497325,15758244,14731276,20974151,30651563