yrhJ

168

cytochrome P450 (CYP102A3)/ NADPH-cytochrome P450 reductase

Locus: BSU_27160

Molecular weight: 118.47 kDa

Isoelectric point: 6.02

Protein length: 1054 aa

Gene length: 3165 bp

Function: fatty acid metabolism

Product: NADPH-cytochrome P450 reductase

Essential: no

E.C.: 1.6.2.4

Synonyms: yrhJ, cypB

Genomic Context

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List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene

Coordinates: 2,773,890  2,777,054

The protein

Catalyzed reaction/ biological activity

• hydroxylates medium-chain fatty acids in subterminal positions PubMed
• organic molecule + O₂ + reduced [NADPH—hemoprotein reductase] --> alcohol + H⁺ + H₂O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
• NADPH + 2 oxidized [cytochrome P450] --> H⁺ + NADP⁺ + 2 reduced [cytochrome P450] (according to UniProt)

Protein family

• N-terminal part: cytochrome P450 family (according to UniProt)

Domains

• Flavodoxin-like domain (aa 486-625) (according to UniProt)
• FAD-binding FR-type domain (aa 663-896) (according to UniProt)

Structure

• 2X7Y (PDB) (from Bacillus megaterium; 67% identity, 88% similarity) PubMed
• O08336 (AlphaFold)

Paralogous protein(s)

• YetO

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: fatR-yrhJ

  Description: PubMed

  Regulation

  • expression is reduced in a SigV mutant PubMed

  Regulatory mechanism

  • FatR: repression, PubMed, in fatR regulon

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon
  • SigW: sigma factor, PubMed, in sigW regulon
  • SigX: sigma factor, PubMed, in sigX regulon

  Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed

• Genes: yrhH-fatR-yrhJ

  Description: PubMed

  Regulation

  • Expressed under stress conditions (SigW, SigX) PubMed

  Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed

Biological materials

Mutant

• MGNA-A163 (yrhJ::erm), available at the NBRP B. subtilis, Japan
• BKE27160 (yrhJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGTTTCATTAACATCTCCC,  downstream forward: _UP4_TAAAATATAAAATCCCGCCA
• BKK27160 (yrhJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGTTTCATTAACATCTCCC,  downstream forward: _UP4_TAAAATATAAAATCCCGCCA

References

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