adhA

168

thiol-dependent aldehyde dehydrogenase, repair of damaged thiol-containing proteins

Locus: BSU_27010

Molecular weight: 37.67 kDa

Isoelectric point: 5.37

Protein length: 349 aa

Gene length: 1050 bp

Function: response to toxic formaldehyde

Product: thiol-dependent aldehyde dehydrogenase, reduction of aldehydes

Essential: no

Synonyms: adhA

Genomic Context

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List of homologs in different organisms, belongs to COG1064 (Galperin et al., 2021)

Gene

Coordinates: 2,756,312  2,757,361

The protein

Protein family

• zinc-containing alcohol dehydrogenase family (according to UniProt)

Structure

• 1UUF (PDB) (from E. coli, 54% identity)
• C0SPA5 (AlphaFold)

Expression and Regulation

Operons

• Genes: yrzP-adhA-yraA

  Description: PubMed

• Genes: adhA-yraA

  Description: PubMed

  Regulation

  • ''adhA'': induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde (AdhR) PubMed

  Regulatory mechanism

  • AdhR: activation, PubMed, in adhR regulon

Biological materials

Mutant

• MGNA-A260 (adhA::erm), available at the NBRP B. subtilis, Japan
• BKE27010 (adhA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGAACCTATCCTTTCT,  downstream forward: _UP4_TAATAGGTTAGTCCTTATAT
• BKK27010 (adhA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACGAACCTATCCTTTCT,  downstream forward: _UP4_TAATAGGTTAGTCCTTATAT

References

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