spoIIIAE

168

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Locus: BSU_24390

Molecular weight: 43.68 kDa

Isoelectric point: 6.59

Protein length: 399 aa

Gene length: 1200 bp

Function: activation of SigG

Product: component of a type III secretion system

Essential: no

Synonyms: spoIIIAE

Genomic Context

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List of homologs in different organisms, belongs to COG5826 (Galperin et al., 2021)

Gene

Coordinates: 2,534,326  2,535,525

The protein

Structure

• P49782 (AlphaFold)

Localization

• membrane protein PubMed, insertion into the membrane requires YidC1 PubMed

Expression and Regulation

Operons

• Genes: yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

Biological materials

Mutant

• BKE24390 (spoIIIAE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCAATCTACCGCCTCCTT,  downstream forward: _UP4_ATGATGAAATGAAGGAGGCA
• BKK24390 (spoIIIAE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCAATCTACCGCCTCCTT,  downstream forward: _UP4_ATGATGAAATGAAGGAGGCA

Labs working on this gene/protein

• Richard Losick, Harvard University, Cambridge, USA homepage
• Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

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Original Publications

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