cgeB

168

[metabolite|UDP-glucose]-dependent glycosyltransferase, maturation of the outermost layer of the spore

locus: BSU_19790

Molecular weight: 36.70 kDa

pI: 6.57

Protein length: 317 aa

Gene length: 954 bp

function: maturation of the outermost layer of the spore

product: [metabolite|UDP-glucose]-dependent glycosyltransferase

essential: no

synonyms: cgeB, cgeAB

List of homologs in different organisms, belongs to COG4641 (Galperin et al., 2021)

Gene

Coordinates: 2,149,084  2,150,037

The protein

Catalyzed reaction/ biological activity

• [metabolite|UDP-glucose] + [protein|9D3D72FDF028A8468A92C583138397A5BD2FED59|cgeA] --> [protein|9D3D72FDF028A8468A92C583138397A5BD2FED59|cgeA]-glucose + [metabolite|UDP]

Structure

• [AF|P42090]

Expression and Regulation

Operons

• genes: [gene|9D3D72FDF028A8468A92C583138397A5BD2FED59|cgeA]-[gene|405FD4EA1A5B2B3512F973981EF62CB37664B5F8|cgeB]

  description: [Pubmed|7592393]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|7592393]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|7592393], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
  • [protein|0D7F5504590512E1598B94DC1734BF8FF67ED994|gerR]: activation, [Pubmed|15621419], in [regulon|protein:0D7F5504590512E1598B94DC1734BF8FF67ED994|gerR regulon]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|7592393,15699190], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• BKE19790 ([gene|405FD4EA1A5B2B3512F973981EF62CB37664B5F8|cgeB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATATACAAGACTTTCATCG,  downstream forward: _UP4_TGAAAAATCGTTCATTGCTA
• BKK19790 ([gene|405FD4EA1A5B2B3512F973981EF62CB37664B5F8|cgeB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATATACAAGACTTTCATCG,  downstream forward: _UP4_TGAAAAATCGTTCATTGCTA

References

• 7592393,15699190,30582883