hfq

168

RNA chaperone

Locus: BSU_17340

Molecular weight: 8.38 kDa

Isoelectric point: 8.7

Protein length: 73 aa

Gene length: 222 bp

Function: unknown

Product: RNA chaperone

Essential: no

Synonyms: hfq, ymaH

Genomic Context

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List of homologs in different organisms, belongs to COG1923 (Galperin et al., 2021)

Gene

Coordinates: 1,867,373  1,867,594

The protein

Protein family

• hfq family (single member, according to UniProt)

Structure

• 3HSB (PDB) (complex with an RNA aptamer) PubMed
• O31796 (AlphaFold)

Expression and Regulation

Operons

• Genes: miaA-hfq

  Description: PubMed

• Genes: gerY-miaA-hfq

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: hfq

  Description: PubMed

  Regulation

  • expression (mRNA levels) is quite constant during growth in minimal medium PubMed

Biological materials

Mutant

• GP22 (hfq::cat), available in Jörg Stülke's lab
• BKE17340 (hfq::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT,  downstream forward: _UP4_TAGATCAAAAAATGCCATGT
• BKK17340 (hfq::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT,  downstream forward: _UP4_TAGATCAAAAAATGCCATGT

Expression vectors

• pGP2166: expression of hfq-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1067 (spc, based on pGP1331), available in Jörg Stülke's lab

LacZ fusion

• pGP460 (in pAC7), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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