rimM

168

16S rRNA processing protein, RNase

Locus: BSU_16020

Molecular weight: 20.06 kDa

Isoelectric point: 5.22

Protein length: 174 aa

Gene length: 525 bp

Function: maturation of 16S rRNA

Product: 16S rRNA processing protein, RNase

Essential: no

Synonyms: rimM, ylqE

Genomic Context

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List of homologs in different organisms, belongs to COG0806 (Galperin et al., 2021)

Gene

Coordinates: 1,674,650  1,675,174

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• defective in 16S rRNA 3' processing by YqfG PubMed

The protein

Protein family

• RimM family (single member, according to UniProt)

Domains

• PRC barrel domain (aa 98-171) (according to UniProt)

Structure

• 3H9N (PDB) (from Haemophilus influenzae, 33% identity)
• O31740 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ylxM-ffh-rpsP-khpA-ylqD-rimM-trmD-rplS

  Description: PubMed

  Regulation

  • the mRNA is processed between ffh and rpsP by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Expression

  • expression is decreased upon depletion of tRNA maturation factors rnpB or rnz PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: rpsP-khpA-ylqD-rimM-trmD-rplS

  Description: PubMed

• Genes: rpsP-khpA-ylqD-rimM

  Description: PubMed

Biological materials

Mutant

• MGNA-B376 (ylqE::erm), available at the NBRP B. subtilis, Japan
• BKE16020 (rimM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGATCACCTCTGGCGA,  downstream forward: _UP4_GAATGAAAATCGATTTTTTG
• BKK16020 (rimM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGATCACCTCTGGCGA,  downstream forward: _UP4_GAATGAAAATCGATTTTTTG

References

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