thiX

168

thiamine [wiki|ECF transporter] (membrane-spanning T protein)

locus: BSU_13210

Molecular weight: 27.89 kDa

pI: 10.02

Protein length: 254 aa

Gene length: 765 bp

function: thiamine uptake

product: thiamine [wiki|ECF transporter] (membrane-spanning T protein)

essential: no

synonyms: thiX, ykoC

List of homologs in different organisms, belongs to COG0619 (Galperin et al., 2021)

Gene

Coordinates: 1,388,070  1,388,834

The protein

Protein family

• cbiQ family (single member, according to UniProt)

Structure

• [AF|O34572]

[wiki|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]

  description: [Pubmed|16291685]

  regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• MGNA-B311 (ykoC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1310 NBRP B. subtilis, Japan]
• BKE13210 ([gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACCGCCGCCGCTTTCACAG,  downstream forward: _UP4_TAAAAAATCCGCTATCACAG
• BKK13210 ([gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACCGCCGCCGCTTTCACAG,  downstream forward: _UP4_TAAAAAATCCGCTATCACAG

References

• 10092453,16291685,12376536,18763711,36815589