proB

168

glutamate 5-kinase

Locus: BSU_13120

Molecular weight: 39.30 kDa

Isoelectric point: 6.01

Protein length: 365 aa

Gene length: 1098 bp

Function: biosynthesis of proline

Product: glutamate 5-kinase

Essential: no

E.C.: 2.7.2.11

Synonyms: proB

Genomic Context

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List of homologs in different organisms, belongs to COG0263 (Galperin et al., 2021)

Gene

Coordinates: 1,378,496  1,379,593

Phenotypes of a mutant

• auxotrophic for proline PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-glutamate --> ADP + L-glutamyl 5-phosphate (according to UniProt)

Protein family

• glutamate 5-kinase family (with ProJ, according to UniProt)

Domains

• PUA domain (aa 276-353) (according to UniProt)

Structure

• 2J5V (PDB) (from E. coli, 40% identity) PubMed
• P39820 (AlphaFold)

Effectors of protein activity

• the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}

Paralogous protein(s)

• ProJ

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: proB-proA

  Description: PubMed

  Regulation

  • induced by proline limitation (T-box) PubMed
  • expression requires functional RnpM PubMed

  Regulatory mechanism

  • T-box: RNA switch, in T-box
  • RnpM: unknown, PubMed, in rnpM regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • ProA is absent from the membrane proteome of a 'BdbC-BdbD' mutant PubMed
  • the T-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• BKE13120 (proB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATTCTCCTCCGCGGC,  downstream forward: _UP4_GTAAAAGACTAGGAGGCGGA
• BKK13120 (proB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTATTCTCCTCCGCGGC,  downstream forward: _UP4_GTAAAAGACTAGGAGGCGGA

References

Reviews

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Original Publications

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