yitC

168

mother cell-specific sporulation protein, similar to 2-phosphosulfolactate phosphatase

Locus: BSU_10940

Molecular weight: 24.51 kDa

Isoelectric point: 5.22

Protein length: 228 aa

Gene length: 687 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yitC

Genomic Context

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List of homologs in different organisms, belongs to COG2045 (Galperin et al., 2021)

Gene

Coordinates: 1,172,650 → 1,173,336

The protein

Catalyzed reaction/ biological activity

• (2R)-O-phospho-3-sulfolactate + H₂O --> (R)-3-sulfolactate + phosphate (according to UniProt)

Protein family

• ComB family (single member, according to UniProt)

Structure

• 1VR0 (PDB) (from Clostridium acetobutylicum, 31% identity) PubMed
• O06738 (AlphaFold)

Additional information

• The gene is annotated in KEGG as ortholog of 2-phosphosulfolactate phosphatase EC 3.1.3.71. In Swiss-Prot the protein is marked as “probable 2-phosphosulfolactate phosphatase”. No EC annotation is available in MeMetaCyc.No literature/experimental evidence supporting the annotation is available. PubMed

Expression and Regulation

Operons

• Genes: yitC-yitD

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-B177 (yitC::erm), available at the NBRP B. subtilis, Japan
• BKE10940 (ΔyitC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTACCTCTCCTTGGGC,  downstream forward: _UP4_CCAACGATAGAGAGGGTCAT
• BKK10940 (ΔyitC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTACCTCTCCTTGGGC,  downstream forward: _UP4_CCAACGATAGAGAGGGTCAT

References

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