yhjQ

168

copper storage protein

Locus: BSU_10600

Molecular weight: 11.70 kDa

Isoelectric point: 4.9

Protein length: 108 aa

Gene length: 327 bp

Function: delivery of copper to Laccase

Product: copper storage protein

Essential: no

Synonyms: yhjQ, csp3

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,135,255 → 1,135,581

The protein

Catalyzed reaction/ biological activity

• binding of Cu(I) ions (up to 80 ions per tetramer) to prevent copper toxicity PubMed

Structure

• 5FIG (PDB) PubMed
• O07571 (AlphaFold)

Expression and Regulation

Operons

• Genes: yhjR-yhjQ

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: repression, PubMed, in gerE regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-B288 (yhjQ::erm), available at the NBRP B. subtilis, Japan
• BKE10600 (ΔyhjQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTTGATTCCCCCTGTA,  downstream forward: _UP4_TAAAAAAGAGGGTTCAAAAA
• BKK10600 (ΔyhjQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTTGATTCCCCCTGTA,  downstream forward: _UP4_TAAAAAAGAGGGTTCAAAAA

References

Reviews

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Original Publications

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