plsC

168

acyl-ACP:1-acylglycerolphosphate acyltransferase

Locus: BSU_09540

Molecular weight: 21.78 kDa

Isoelectric point: 9.98

Protein length: 199 aa

Gene length: 600 bp

Function: biosynthesis of phospholipids

Product: acyl-ACP:1-acylglycerolphosphate acyltransferase

Essential: yes

E.C.: 2.3.1.51

Synonyms: plsC, yhdO

Genomic Context

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List of homologs in different organisms, belongs to COG0204 (Galperin et al., 2021)

Gene

Coordinates: 1,031,395 → 1,031,994

Phenotypes of a mutant

• essential PubMed
• specific point mutations affecting PlsC (R48P, A61V, or P110S) facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate, especially in combination with YfkC-R42W PubMed

The protein

Catalyzed reaction/ biological activity

• Acyl-AcpA + 1-acyl-sn-glycerol 3-phosphate --> AcpA + 1,2-diacyl-sn-glycerol 3-phosphate (according to UniProt)

Protein family

• 1-acyl-sn-glycerol-3-phosphate acyltransferase family (single member, according to UniProt)

Structure

• 5KYM (PDB) (from Thermotoga maritima) PubMed
• O07584 (AlphaFold)

Localization

• cell membrane at the septum PubMed

Expression and Regulation

Operons

• Genes: yhdN-plsC

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Regulatory mechanism

  • FapR: repression, PubMed, in fapR regulon

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-B483 (yhdO::erm), available at the NBRP B. subtilis, Japan
• GP3131 (yfkC-R42W kan plsC-L48P phleo) , available in Jörg Stülke's lab

FLAG-tag construct

• GP3683 (spc, based on pGP1331), available in Jörg Stülke's lab

lacZ fusion

• GP3674 (based on pAC7), available in Jörg Stülke's lab

References

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