bsuMA

168

DNA-3-methyladenine glycosidase II

Locus: BSU_06060

Molecular weight: 48.75 kDa

Isoelectric point: 7.66

Protein length: 427 aa

Gene length: 1284 bp

Function: BsuM DNA modification

Product: DNA-3-methyladenine glycosidase

Essential: yes

Synonyms: ydiO

Genomic Context

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Categories containing this gene/protein

Information processing, Genetics, DNA restriction/ modification

Prophages and mobile genetic elements, Prophages, Prophage 3

Groups of genes, Essential genes

List of homologs in different organisms, belongs to COG0270 (Galperin et al., 2021)

This gene is a member of the following regulons

LexA regulon

Gene

Coordinates: 655,223  656,506

Phenotypes of a mutant

essential PubMed, mutation is only possible in a ''bsuRA, bsuRB, or bsuRC background (i. e.'', in the absence of the BsuM DNA restriction system) PubMed

The protein

Catalyzed reaction/ biological activity

2'-deoxycytidine in DNA + S-adenosyl-L-methionine --> 5-methyl-2'-deoxycytidine in DNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

Methyltransferase superfamily (according to UniProt)
class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Domains

SAM-dependent MTase C5-type domain (aa 84-427) (according to UniProt)

Structure

3G7U (PDB) (from E. coli phage, 28% identity)
O34939 (AlphaFold)

Expression and Regulation

Operons

Genes: bsuMA-bsuMB

Description: PubMed

Regulation

induced by DNA damage (LexA) PubMed

Regulatory mechanism

LexA: repression, PubMed, in lexA regulon

References

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