trmN6

168

tRNA methyltransferase

Locus: BSU_00340

Molecular weight: 28.13 kDa

Isoelectric point: 6.5

Protein length: 247 aa

Gene length: 744 bp

Function: tRNA modification

Product: tRNA methyltransferase

Essential: no

Synonyms: trmN6, yabB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4123 (Galperin et al., 2021)

Gene

Coordinates: 42,917  43,660

The protein

Structure

• 3LPM (PDB) (putative methyltransferase from Listeria monocytogenes, 64% identity)
• P37543 (AlphaFold)

Expression and Regulation

Operons

• Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

• Genes: darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

  Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

• Genes: tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

Biological materials

Mutant

• MGNA-B901 (yabB::erm), available at the NBRP B. subtilis, Japan
• BKE00340 (trmN6::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCTTATCCTTTCAGT,  downstream forward: _UP4_ACAAAAGAAATCAGGACCAT
• BKK00340 (trmN6::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCTTATCCTTTCAGT,  downstream forward: _UP4_ACAAAAGAAATCAGGACCAT

References

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