YcbF

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  • Description: glucarate dehydratase

Gene name ycbF
Synonyms gudD
Essential no
Product glucarate dehydratase
Function glucarate utilization
MW, pI 50 kDa, 5.594
Gene length, protein length 1365 bp, 455 aa
Immediate neighbours ycbE, ycbG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YcbF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU02490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: d-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O (according to Swiss-Prot)
  • Protein family: GlucD subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: induced by D-glucarate/galactarate (YcbG) PubMed
  • Regulatory mechanism: YcbG: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shigeo Hosoya, Kunio Yamane, Michio Takeuchi, Tsutomu Sato
Identification and characterization of the Bacillus subtilis D-glucarate/galactarate utilization operon ycbCDEFGHJ.
FEMS Microbiol Lett: 2002, 210(2);193-9
[PubMed:12044674] [WorldCat.org] [DOI] (P p)

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed