GlnR

• Description: transcriptional repressor of the glnR-glnA operon
  
  

Gene name	glnR
Synonyms
Essential	no
Product	transcription repressor
Function	regulation of glutamine synthesis
Metabolic function and regulation of this protein in SubtiPathways: Cell wall
MW, pI	15 kDa, 9.731
Gene length, protein length	405 bp, 135 aa
Immediate neighbours	ynbB, glnA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU17450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): feedback-inhibited GlnA

• Effectors of protein activity:

• Interactions: GlnR-GlnA, with feedback-inhibited GlnA, this results in DNA binding

• Localization:

Database entries

• Structure:

• Swiss prot entry: P37582

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glnR-glnA PubMed

• Sigma factor:

• Regulation: expressed in the absence of glutamine PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism: repressed by GlnR-GlnA complex PubMed
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Susan H Fisher, Lewis V Wray
Novel trans-Acting Bacillus subtilis glnA mutations that derepress glnRA expression.
J Bacteriol: 2009, 191(8);2485-92
[PubMed:19233925] [WorldCat.org] [DOI] (I p)

Susan H Fisher, Lewis V Wray
Bacillus subtilis glutamine synthetase regulates its own synthesis by acting as a chaperone to stabilize GlnR-DNA complexes.
Proc Natl Acad Sci U S A: 2008, 105(3);1014-9
[PubMed:18195355] [WorldCat.org] [DOI] (I p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

S W Brown, A L Sonenshein
Autogenous regulation of the Bacillus subtilis glnRA operon.
J Bacteriol: 1996, 178(8);2450-4
[PubMed:8636055] [WorldCat.org] [DOI] (P p)

H J Schreier, S W Brown, K D Hirschi, J F Nomellini, A L Sonenshein
Regulation of Bacillus subtilis glutamine synthetase gene expression by the product of the glnR gene.
J Mol Biol: 1989, 210(1);51-63
[PubMed:2573733] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed