HemC

• Description: hydroxymethylbilane synthase
  
  

Gene name	hemC
Synonyms
Essential	no
Product	hydroxymethylbilane synthase
Function	heme biosynthesis
MW, pI	34 kDa, 5.088
Gene length, protein length	942 bp, 314 aa
Immediate neighbours	hemD, hemX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28150

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 4 porphobilinogen + H₂O = hydroxymethylbilane + 4 NH₃ (according to Swiss-Prot)

• Protein family: HMBS family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P16616

• KEGG entry: [3]

• E.C. number: 2.5.1.61

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed in the absence of hydrogen peroxide (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

A F Herbig, J D Helmann
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.
Mol Microbiol: 2001, 41(4);849-59
[PubMed:11532148] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed