SpoIIIJ

• Description: membrane protein translocase, essential for sigma-G activity at stage III
  
  

Gene name	spoIIIJ
Synonyms	spo0J87
Essential	no
Product	membrane protein translocase
Function	control of SigG activity
MW, pI	29 kDa, 9.992
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	jag, rnpA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU41040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: membrane protein translocase, facilitates insertion of SpoIIIAE into the membrane PubMed

• Protein family: Type 2 subfamily (according to Swiss-Prot)

• Paralogous protein(s): YqjG, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIIIAE-SpoIIIJ PubMed

• Localization: membrane PubMed

Database entries

• Structure:

• Swiss prot entry: Q01625

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA homepage

Adriano Henriques, Lisbon, Portugal homepage

References

1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
2. Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., and Czaplewski, L. (2007) Essential bacterial functions encoded by gene pairs. J Bacteriol 189, 591-602. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed